3D Spheroid Cultures for Salivary Gland Tissue Engineering: Effects of Fibroblast on Epithelial Cell Function

3D Spheroid Cultures for Salivary Gland Tissue Engineering: Effects of Fibroblast on Epithelial Cell Function

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Three-dimensional (3D) spheroid cultures are crucial for modeling salivary gland (SG) WHOLE RYE morphogenesis and advancing regenerative medicine.This study evaluated the effects of varying ratios of mouse SG-derived epithelial cells co-cultured with human dermal fibroblasts (hDFs), identifying a 2:1 ratio (spheroids containing 67% EpCAMpos cells with 33% hDFs) as optimal for preserving native SG-derived epithelial cell phenotypes.At this ratio, 67% EpCAMpos spheroids maintained structural integrity and demonstrated a significant reduction in apoptosis and senescence markers, specifically, cleaved caspase-3 (Cc3) and Serpine1, alongside Swim One-Piece an enhanced expression of the progenitor marker Keratin 5 (KRT5).This highlights the pivotal role of fibroblasts in supporting epithelial cell function in 3D cultures.These spheroids provide a useful model for developing SG tissues that closely mimic physiological properties.

Despite promising results, these findings are preliminary and require further validation under diverse conditions and across different SG models.